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Temporal and spatial stability of the EM/PM molecular subtypes in adult diffuse glioma

《医学前沿（英文）》 2023年第17卷第2期 页码 240-262 doi: 10.1007/s11684-022-0936-z

摘要： Detailed characterizations of genomic alterations have not identified subtype-specific vulnerabilities in adult gliomas. Mapping gliomas into developmental programs may uncover new vulnerabilities that are not strictly related to genomic alterations. After identifying conserved gene modules co-expressed with EGFR or PDGFRA (EM or PM), we recently proposed an EM/PM classification scheme for adult gliomas in a histological subtype- and grade-independent manner. By using cohorts of bulk samples, paired primary and recurrent samples, multi-region samples from the same glioma, single-cell RNA-seq samples, and clinical samples, we here demonstrate the temporal and spatial stability of the EM and PM subtypes. The EM and PM subtypes, which progress in a subtype-specific mode, are robustly maintained in paired longitudinal samples. Elevated activities of cell proliferation, genomic instability and microenvironment, rather than subtype switching, mark recurrent gliomas. Within individual gliomas, the EM/PM subtype was preserved across regions and single cells. Malignant cells in the EM and PM gliomas were correlated to neural stem cell and oligodendrocyte progenitor cell compartment, respectively. Thus, while genetic makeup may change during progression and/or within different tumor areas, adult gliomas evolve within a neurodevelopmental framework of the EM and PM molecular subtypes. The dysregulated developmental pathways embedded in these molecular subtypes may contain subtype-specific vulnerabilities.

关键词： glioma progression molecular classification EM/PM subtyping intratumor heterogeneity

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血清Nem>-聚糖生物标志物诊断ALT水平正常慢性乙型肝炎患者显著肝纤维化和肝硬化的临床意义 Article

王林, 刘艺琪, 顾启馨, 张驰, 徐蕾, 王蕾, 陈翠英, 刘学恩, 赵鸿, 庄辉

《工程（英文）》 2023年第26卷第7期 页码 151-158 doi: 10.1016/j.eng.2023.03.008

摘要：
本研究目的是探讨血清Nem>-聚糖模型在285例丙氨酸转移酶（alanine aminotransferase, ALT）水平正常应用基于DNA测序仪的荧光糖电泳技术检测患者血清Nem>-聚糖图谱，每例患者的血清样本中共鉴定出9个Nem>-聚糖峰。），并比较血清Nem>-聚糖模型和其他纤维化标志物的诊断效能。在诊断肝硬化（≥F5）时，血清Nem>-聚糖RF-B模型的AUROC为0.97，与肝组织活检的符合率为88.94%。在ALT水平正常的慢性乙肝患者中，血清Nem>-聚糖模型可作为诊断显著肝纤维化或肝硬化的潜在生物标志物。

关键词：肝纤维化 慢性乙型肝炎 血清Nem>-聚糖 Nem>-聚糖模型 丙氨酸转移酶

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Flow cytometric assessment of the effects of chlorine, chloramine, and UV on bacteria by using nucleic acid stains and 5-cyano-2,3-ditolyltetrazolium chloride

Xuebiao Nie, Wenjun Liu, Mo Chen, Minmin Liu, Lu Ao

《环境科学与工程前沿（英文）》 2016年第10卷第6期 doi: 10.1007/s11783-016-0884-4

摘要： Flow cytometry based on nucleic acid stains and CTC was established and optimized. Membrane of is more resistant to chlorine/chloramine than . The metabolic activity of bacteria persisted after the cytomembranewas damaged. showed more resistance to UV irradiation than by FCM. MP-UV was a stronger inhibitor of metabolic activity than LP-UV. Flow cytometry (FCM) has been widely used in multi-parametric assessment of cells in various research fields, especially in environmental sciences. This study detected the metabolic activity of and by using an FCM method based on 5-cyano-2,3-ditolyltetrazolium chloride (CTC); the accuracy of this method was enhanced by adding SYTO 9 and 10% R2A broth. The disinfection effects of chlorine, chloramine, and UV were subsequently evaluated by FCM methods. Chlorine demonstrated stronger and faster destructive effects on cytomembrane than chloramine, and nucleic acids decomposed afterwards. The metabolic activity of the bacteria persisted after the cytomembranewas damaged as detected using CTC. Low-pressure (LP) UV or medium-pressure (MP) UV treatments exerted no significant effects on membrane permeability. The metabolic activity of the bacteria decreased with increasing UV dosage, and MP-UV was a stronger inhibitor of metabolic activity than LP-UV. Furthermore, the membrane of Gram-positive was more resistant to chlorine/chloramine than that of Gram-negative . In addition, showed higher resistance to UV irradiation than .

关键词： Flow cytometry Escherichia coli Staphylococcusaureus UV CTC SYTO 9

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一种融合多特征聚类与神经网络的PM_2.5小时浓度预测新模型及其在中国城市的应用 Article

刘辉, 龙治豪, 段铸, 施惠鹏

《工程（英文）》 2020年第6卷第8期 页码 944-956 doi: 10.1016/j.eng.2020.05.009

摘要：
PM_2.5浓度预测对空气污染物早期预警具有重要意义。在分解部分，提出了一种由粗糙集属性约简(RSAR)、kem>均值聚类(KC)和经验小波变换(EWT)组成的MCD方法进行特征选择和数据分类。在MCD方法中，采用RSAR算法选择重要的空气污染物变量，使用KC算法对所得变量进行聚类，利用EWT算法将PM_2.5浓度序列的聚类结果分解为多个子层。利用我国4个不同城市的真实PM_2.5浓度数据，验证了所提出模型的有效性。实验结果表明，所提出预测模型适用于PM_2.5浓度的多步高精度预测，具有比基准模型更优的预测性能。

关键词： PM_2.52.5浓度预测 PM_2.52.5浓度聚类 经验小波分解 多步预测

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Diverse bacterial populations of PM in urban and suburb Shanghai, China

《环境科学与工程前沿（英文）》 2021年第15卷第3期 doi: 10.1007/s11783-020-1329-7

摘要：
• Urban aerosols harbour diverse bacterial communities in Shanghai.

关键词： PM_2.5 Bacteria 16S rRNA SEM analysis Shanghai City

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结直肠癌、基质和正常结肠黏膜显微解剖区域Nem>-糖组的显著多样性 Article

Di Wang, Katarina Madunić , Tao Zhang, Guinevere S.M. Lageveen-Kammeijer, Manfred Wuhrer

《工程（英文）》 2023年第26卷第7期 页码 32-43 doi: 10.1016/j.eng.2022.08.016

摘要：
Aberrant glycosylation is considered to be a hallmark of colorectal cancer (CRC), as demonstrated by various studies. While the N-glycosylation of cell lines and serum has been widely examined, the analysis of cancer-associated N-glycans from tissues has been hampered by the heterogeneity of tumors and the complexity of N-glycan structures. To overcome these obstacles, we present a study using laser capture microdissection that makes it possible to largely deconvolute distinct N-glycomic signatures originating from different regions of heterogeneous tissues including cancerous, stromal, and healthy mucosa cells. N-glycan alditols were analyzed by means of porous graphitized carbon liquid chromatography-electrospray ionization tandem mass spectrometry, enabling the differentiation and structural characterization of isomeric species. In total, 116 N-glycans were identified that showed profound differences in expression among cancer, stroma, and normal mucosa. In comparison with healthy mucosa, the cancer cells showed an increase in α2-6 sialylation and monoantennary N-glycans, as well as a decrease in bisected N-glycans. Moreover, specific sialylated and (sialyl-)LewisA/X antigen-carrying N-glycans were exclusively expressed in cancers. In comparison with cancer, the stroma showed lower levels of oligomannosidic and monoantennary N-glycans, LewisA/X epitopes, and sulfation, as well as increased expression of (core-)fucosylation and α2-3 sialylation. Our study reveals the distinct N-glycomic profiles of different cell types in CRC tumor and control tissues, proving the necessity of their separate analysis for the discovery of cancer-associated glycans.

关键词：结直肠癌 肿瘤 多孔石墨化碳液相色谱-质谱 Nem>-糖组 抗体反应

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Temporal variation of PM-associated health effects in Shijiazhuang, Hebei

《环境科学与工程前沿（英文）》 2021年第15卷第5期 doi: 10.1007/s11783-020-1376-0

摘要：
•Annual mean PM_2.5 in Shijiazhuang were 87, 95, and 82 µg/m³ in 2015–2017.

关键词： PM_2.5 Health effects Integrated exposure-response model Shijiazhuang

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抗白粉病基因 Pm40 在我国“后 Pm21 时代”小麦育种中的重要作用 Perspective

唐圣雯,胡玉亭,钟胜福,罗培高

《工程（英文）》 2018年第4卷第4期 页码 500-506 doi: 10.1016/j.eng.2018.06.004

摘要： 20 世纪80 年代，通过簇毛麦（Heuchera villosaem>）的6VS 染色体与小麦6AL 染色体易位将抗白粉病基因Pm21 em>转移到普通小麦中。最近，在一些地方发现了对Pm21 em>有毒的Bgtem>，虽然这些菌株的病理学特性还有待研究，但这一现象提醒小麦育种者应注意应用Pm21 em>的风险。通过细胞学研究，并未在Pm40 em>的载体品种中发现大片段外缘染色体。过去几年的研究发现，Pm40 em>的载体品种具有优良的农艺性状。因此，我们相信在未来的育种工程中，Pm40em> 将会在Pm21 em>的抗性被克服之后起巨大的作用。另外，Pm21 em>和Pm40em> 都来源于外缘物种，这暗示着外源基因的抗性可能比小麦本身的基因更为持久和有效。

关键词：小麦 白粉病 Pm21 Pm40 外源基因 内源抗性

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Local and regional contributions to PM in the Beijing 2022 Winter Olympics infrastructure areas during

《环境科学与工程前沿（英文）》 2021年第15卷第6期 doi: 10.1007/s11783-021-1434-2

摘要：
• Regional transportation contributed more than local emissions during haze episodes.

关键词： 2022 Winter Olympics PM_2.5 Source apportionment

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VITHM1 mediated green synthesis of silver nanoparticles: Mechanism and biological applications

Mani Abirami, Krishnan Kannabiran

《化学科学与工程前沿（英文）》 2016年第10卷第4期 页码 542-551 doi: 10.1007/s11705-016-1599-6

摘要： We present the microbial green synthesis of silver nanoparticles (NPs) by VITHM1 strain (MTCC No. 12465). The secondary metabolites in the cell free supernatant of this bacterium when incubated with 1 mmol/L AgNO , mediated the biological synthesis of AgNPs. The synthesized AgNPs were characterized by UV-visible spectrum, X-ray diffraction (XRD), atomic force microscope, scanning electron microscopy equipped with energy dispersive spectroscopy, transmission electron microscopy, FT-IR spectroscopy, dynamic light scattering and zeta potential. They were highly stable and, spherical in shape with the average size of 30?50 nm. The secondary metabolites involved in the formation of AgNPs were identified gas chromatography-mass spectrography. The 3D structure of the unit cell of the synthesized AgNPs was determined using XRD data base. The synthesized AgNPs exhibited significant antibacterial activity against tested bacterial pathogens, and did not show haemolysis on human red blood cells. This green synthesis could provide a new platform to explore and use AgNPs as antibacterial therapeutic agents.

关键词： Streptomyces ghanaensis VITHM1 nanoparticles 3D structure antibacterial activity

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Enhanced disinfection of Escherichia coliem> and bacteriophage

Guiying RAO, Kristen S. BRASTAD, Qianyi ZHANG, Rebecca ROBINSON, Zhen HE, Ying LI

《环境科学与工程前沿（英文）》 2016年第10卷第4期 doi: 10.1007/s11783-016-0854-x

摘要： A novel photocatalytic Ag-Cu-TiO nanowire membrane was fabricated. Bacteria and virus disinfection was improved by co-depositing Ag and Cu onto membrane. Synergetic photocatalytic effects and free metal ions of Ag and Cu contribute to disinfection. 7.68 log removal of and 4.02 log removal of bacteriophage MS2 were achieved. Titanium dioxide (TiO ) is a widely used photocatalyst that has been demonstrated for microorganism disinfection in drinking water. In this study, a new material with a novel structure, silver and copper loaded TiO nanowire membrane (Cu-Ag-TiO ) was prepared and evaluated for its efficiency to inactivate and bacteriophage MS2. Enhanced photo-activated bactericidal and virucidal activities were obtained by the Cu-Ag-TiO membrane than by the TiO , Ag-TiO and Cu-TiO membranes under both dark and UV light illumination. The better performance was attributed to the synergies of enhanced membrane photoactivity by loading silver and copper on the membrane and the synergistic effect between the free silver and copper ions in water. At the end of a 30 min test of dead-end filtration under 254 nm UV irradiation, the Cu-Ag-TiO membrane was able to obtain an removal of 7.68 log and bacteriophage MS2 removal of 4.02 log, which have met the US EPA standard. The free metal ions coming off the membrane have concentrations of less than 10 ppb in the water effluent, far below the US EPA maximum contaminant level for silver and copper ions in drinking water. Therefore, the photo-activated disinfection by the Cu-Ag-TiO membrane is a viable technique for meeting drinking water treatment standards of microbiological water purifiers.

关键词： Photo-activated disinfection Titanium dioxide Nanowire membrane Silver Copper

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可去除染料——Nem>-聚糖多方法深入分析中的缺失环节 Article

Samanta Cajic, René Hennig, Valerian Grote, Udo Reichl, Erdmann Rapp

《工程（英文）》 2023年第26卷第7期 页码 132-150 doi: https://doi.org/10.1016/j.eng.2023.02.016

摘要：
As the roles of glycans in health and disease continue to be unraveled, it is becoming apparent that glycans' immense complexity cannot be ignored. To fully delineate glycan structures, we developed an integrative approach combining a set of cost-effective, widespread, and easy-to-handle analytical methods. The key feature of our workflow is the exploitation of a removable fluorescent label—exemplified by 9-fluorenylmethyl chloroformate (Fmoc)—to bridge the gap between diverse glycoanalytical methods, especially multiplexed capillary gel electrophoresis with laser-induced fluorescence detection (xCGE-LIF) and matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF-MS). Through the detailed structural analysis of selected, dauntingly complex N-glycans from chicken ovalbumin, horse serum, and bovine transferrin, we illustrate the capabilities of the presented strategy. Moreover, this approach "visualizes" N-glycans that have been difficult to identify thus far—such as the sulfated glycans on human immunoglobulin A—including minute changes in glycan structures, potentially providing useful new targets for biomarker discovery.

关键词：糖蛋白 N-聚糖 可逆标签 亲水相互作用液相色谱法 毛细管凝胶电泳 质谱法

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基于正交质谱的Nem>-糖组谱揭示哈夫病潜在病原学 Article

刘思, 刘圆圆, 林佳静, 刘笔锋, 何振宇, 吴晓旻, 刘欣

《工程（英文）》 2023年第26卷第7期 页码 63-73 doi: 10.1016/j.eng.2022.09.012

摘要： Nem>-糖组的剖析将促进破译疾病的分子机制，而HD相关的糖基化从未被探索过。本文中，采用基于高通量的正交质谱对HD中血清和血清衍生的IgG的Nem>-糖组谱进行了表征。数据显示，HD与总血清糖蛋白的核心岩藻糖基化和单半乳糖醇化升高有关。本研究表明差异化IgG Nem>-糖基化与HD的关联，为这种罕见疾病的病因提供了新的见解。

关键词：哈夫病 全血清 IgG抗体 糖基化 疾病病原学

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PM2.5 over North China based on MODIS AOD and effect of meteorological elements during 2003‒2015

Youfang Chen, Yimin Zhou, Xinyi Zhao

《环境科学与工程前沿（英文）》 2020年第14卷第2期 doi: 10.1007/s11783-019-1202-8

摘要： The Taihang Mountains was the boundary between high and low pollution areas. There were one high value center for PM2.5 pollution and two low value centers. In 2004, 2009 and after 2013, PM2.5 concentration was relatively low. Over the past 40 years, PM2.5 pollution in North China has become increasingly serious and progressively exposes the densely populated areas to pollutants. However, due to limited ground data, it is challenging to estimate accurate PM2.5 exposure levels, further making it unfavorable for the prediction and prevention of PM2.5 pollutions. This paper therefore uses the mixed effect model to estimate daily PM2.5 concentrations of North China between 2003 and 2015 with ground observation data and MODIS AOD satellite data. The tempo-spatial characteristics of PM2.5 and the influence of meteorological elements on PM2.5 is discussed with EOF and canonical correlation analysis respectively. Results show that overall R2 is 0.36 and the root mean squared predicted error was 30.1 μg/m3 for the model prediction. Our time series analysis showed that, the Taihang Mountains acted as a boundary between the high and low pollution areas in North China; while the northern part of Henan Province, the southern part of Hebei Province and the western part of Shandong Province were the most polluted areas. Although, in 2004, 2009 and dates after 2013, PM2.5 concentrations were relatively low. Meteorological/topography conditions, that include high surface humidity of area in the range of 34°‒40°N and 119°‒124°E, relatively low boundary layer heights, and southerly and easterly winds from the east and north area were common factors attributed to haze in the most polluted area. Overall, the spatial distribution of increasingly concentrated PM2.5 pollution in North China are consistent with the local emission level, unfavorable meteorological conditions and topographic changes.

关键词： Aerosol optical depth PM_2.5 MODIS Mixed effect model Canonical correlation analysis

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燃煤电站烟气污染物深度脱除技术的分析

张晓鲁

《中国工程科学》 2014年第16卷第10期 页码 47-51

摘要：
近年来雾霾天气的频繁出现使得细颗粒物（PM_2.5）成为了公众关注的热点，PM2.5的控制也已增加到2012 年发布的《环境空气质量标准》中，而目前我国现有的烟气污染物控制技术难以脱除PM_2.5，因此，为深度脱除PM_2.5、SO₂、SO_{3PM_2.5脱除技术、全负荷下超超临界锅炉的低NO_x排放以及SCR工作温度的适应性}

关键词：燃煤机组 烟气污染物 深度脱除 PM2.5脱除

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标题作者时间类型操作

Temporal and spatial stability of the EM/PM molecular subtypes in adult diffuse glioma

期刊论文



血清Nem>-聚糖生物标志物诊断ALT水平正常慢性乙型肝炎患者显著肝纤维化和肝硬化的临床意义

王林, 刘艺琪, 顾启馨, 张驰, 徐蕾, 王蕾, 陈翠英, 刘学恩, 赵鸿, 庄辉

期刊论文



Flow cytometric assessment of the effects of chlorine, chloramine, and UV on bacteria by using nucleic acid stains and 5-cyano-2,3-ditolyltetrazolium chloride

Xuebiao Nie, Wenjun Liu, Mo Chen, Minmin Liu, Lu Ao

期刊论文



一种融合多特征聚类与神经网络的PM_2.5小时浓度预测新模型及其在中国城市的应用

刘辉, 龙治豪, 段铸, 施惠鹏

期刊论文



Diverse bacterial populations of PM in urban and suburb Shanghai, China

期刊论文



结直肠癌、基质和正常结肠黏膜显微解剖区域Nem>-糖组的显著多样性

Di Wang, Katarina Madunić , Tao Zhang, Guinevere S.M. Lageveen-Kammeijer, Manfred Wuhrer

期刊论文



Temporal variation of PM-associated health effects in Shijiazhuang, Hebei

期刊论文



抗白粉病基因 Pm40 在我国“后 Pm21 时代”小麦育种中的重要作用

唐圣雯,胡玉亭,钟胜福,罗培高

期刊论文



Local and regional contributions to PM in the Beijing 2022 Winter Olympics infrastructure areas during

期刊论文



VITHM1 mediated green synthesis of silver nanoparticles: Mechanism and biological applications

Mani Abirami, Krishnan Kannabiran

期刊论文



Enhanced disinfection of Escherichia coliem> and bacteriophage

Guiying RAO, Kristen S. BRASTAD, Qianyi ZHANG, Rebecca ROBINSON, Zhen HE, Ying LI

期刊论文



可去除染料——Nem>-聚糖多方法深入分析中的缺失环节

Samanta Cajic, René Hennig, Valerian Grote, Udo Reichl, Erdmann Rapp

期刊论文



基于正交质谱的Nem>-糖组谱揭示哈夫病潜在病原学

刘思, 刘圆圆, 林佳静, 刘笔锋, 何振宇, 吴晓旻, 刘欣

期刊论文



PM2.5 over North China based on MODIS AOD and effect of meteorological elements during 2003‒2015

Youfang Chen, Yimin Zhou, Xinyi Zhao

期刊论文



燃煤电站烟气污染物深度脱除技术的分析

张晓鲁

期刊论文